bch 311 tHEORY qUESTIONS: Titration Analysis of Carbonic Acid (H₂CO₃): Buffering Regions, Midpoint Species, and Henderson-Hasselbalch Equation

300 Level Biochemistry Department exam questions and detailed answers. Download the answers in document format. Correct, detailed, and straightforward answers.

Questions:

1. Assuming a diprotic acid, H2CO3H2​CO3​ (carbonic acid, pK1=6.35pK1​​=6.35 and pK2=10.3pK2​​=10.3).
a. Draw a titration curve reflecting the pattern of H2CO3H2​CO3​ as a strong base is added.
b. Identify the regions (pH ranges) where buffering occurs.
c. Identify the chemical species at the midpoint of the two phases in the titration curve.
d. Derive the Henderson-Hasselbalch equation and state its significance.”

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